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Biology Exam Review
A practice exam with questions from all 5 units, then the key facts from every lesson, unit by unit. For a deeper look at one unit, open its test review.
Unit test reviews
Practice exam
35 questions, the same number from each unit. Each one shows the lesson it comes from.
- Unit 1: Classification and Taxonomy1Which scientific name is written correctly?
- Unit 1: Dichotomous Keys and Field Sampling2Which couplet is best for a dichotomous key?
- Unit 1: Prokaryotes, Eukaryotes and Viruses3Which observation most strongly supports the idea that chloroplasts were once free-living bacteria?
- Unit 1: Protists and Fungi4Put the steps of fungal feeding in order.
- 1Absorbed nutrients fuel growth of more hyphae
- 2Small molecules are absorbed through the hyphal walls
- 3Large molecules are broken down outside the hyphae
- 4Hyphae grow into the food source
- 5Hyphae release digestive enzymes
- Unit 1: From Algae to Flowers: Plant Diversity and Evolution5Why could seed plants spread into dry habitats where ferns struggle?
- Unit 1: The Animal Kingdom: Invertebrates to Vertebrates6Match each body-cavity type to an example phylum.AcoelomatePseudocoelomateCoelomate
- Unit 1: Biodiversity and Human Impact7Which conservation strategy protects all three levels of biodiversity at once?
- Unit 2: The Theory of Evolution and Its Evidence8Which idea from Charles Lyell was most important to Darwin's thinking?
- Unit 2: The Theory of Evolution and Its Evidence9Why is DNA comparison considered especially strong evidence for evolution?
- Unit 2: Natural Selection and Adaptation10Which statement about antibiotic resistance is correct?
- Unit 2: Mechanisms of Evolutionary Change11Why does genetic drift have a much stronger effect in small populations?
- Unit 2: Selective Breeding: Benefits and Trade-offs12The cross that produced Marquis wheat was made in 1892, and the variety was released to farmers in about 1909. How many years did the breeding program take? Give a whole number.
- Unit 2: Speciation and Extinction13According to the biological species concept, two organisms belong to the same species if they:
- Unit 2: Speciation and Extinction14Which species is most likely to be vulnerable to extinction if its habitat changes rapidly?
- Unit 3: DNA, Genes, Chromosomes and Mitosis15Arrange the levels of genetic organization from smallest to largest.
- 1Gene
- 2Genome
- 3Chromosome
- 4Nucleotide
- Unit 3: Meiosis16In which stage do homologous chromosomes separate and move to opposite poles?
- Unit 3: Mendelian Inheritance17A pea plant has the genotype , where (tall) is dominant to (short). Which statement is correct?
- Unit 3: Beyond Simple Dominance18A red bull and a white cow have a calf whose coat has a mix of red hairs and white hairs. What pattern of inheritance does this show?
- Unit 3: Beyond Simple Dominance19Match each example to its pattern of inheritance.Red and white snapdragons produce pink offspringType AB blood shows both A and B markersA carrier mother passes a trait to half her sonsTwo genes give a 9 : 3 : 3 : 1 phenotypic ratio
- Unit 3: Gene Mutations and Single-Gene Disorders20SemBioSys Genetics of Calgary engineered which organism to produce human insulin?
- Unit 3: Chromosomal Disorders and Genetic Technologies21What is the name for a picture of an individual's chromosomes arranged in homologous pairs by size and banding pattern?
- Unit 4: The Digestive System22Where does the chemical digestion of protein begin?
- Unit 4: The Respiratory System23What does the diaphragm do during inhalation?
- Unit 4: The Circulatory System24Which chamber of the heart has the thickest muscular wall?
- Unit 4: Dissection: How the Systems Fit Together25In a fetal pig, which term describes the position of the kidneys relative to the intestines?
- Unit 4: Dissection: How the Systems Fit Together26Which action is an example of the "Replace" principle of the Three Rs?
- Unit 4: Breathing, Circulation and Digestion Across the Animal Kingdom27Why is a four-chambered heart linked to endothermy in birds and mammals?
- Unit 4: System Disorders and Medical Technology28Match each technology to how it works.MRICTUltrasoundInsulin pump
- Unit 5: Plant Tissues and Structures29Which tissue produces the new cells that make a root grow longer?
- Unit 5: Plant Tissues and Structures30Put these leaf layers in order from the top surface of the leaf to the bottom surface.
- 1Lower epidermis
- 2Spongy mesophyll
- 3Palisade mesophyll
- 4Cuticle
- 5Upper epidermis
- Unit 5: Transport in Plants31At 400×, a microscope's field of view is 0.50 mm in diameter. A leaf peel shows 30 stomata in one field of view. What is the stomatal density, in stomata per mm², to the nearest whole number?stomata/mm²
- Unit 5: Plant Growth and Reproduction32Which hormone is mainly responsible for a shoot bending toward light?
- Unit 5: Plant Growth and Reproduction33Corn has a diploid chromosome number of 2n = 20. How many chromosomes are in each cell of a corn kernel's endosperm?chromosomes
- Unit 5: Plants, Sustainability and Society34In the Haudenosaunee Three Sisters planting, what is the main role of the squash?
- Unit 5: Plants, Sustainability and Society35Match each medicine to the plant it was first derived from.ASA (aspirin)MorphineQuininePaclitaxelDigoxin
Key facts by unit
Unit 1: Diversity of Living Things
Classification and Taxonomy
- Ranks from broadest to most specific: domain, kingdom, phylum, class, order, family, genus, species.
- Binomial name = Genus (capitalized) + specific epithet (lowercase), italicized or underlined: Homo sapiens.
- Three domains: Bacteria and Archaea (prokaryotic) and Eukarya (eukaryotic). Archaea are more closely related to Eukarya than to Bacteria.
- Six kingdoms: Eubacteria, Archaebacteria, Protista, Fungi, Plantae, Animalia.
- The lower the rank two organisms share, the more closely related they are.
- Cladograms group organisms by shared derived characteristics; each node is a common ancestor.
Dichotomous Keys and Field Sampling
- A dichotomous key offers two contrasting choices at each step; a key for organisms needs couplets.
- Good couplets use observable, stable features, stated as true opposites.
- Population density:
- Estimated population: total habitat area.
- Random placement and many replicate samples reduce bias and error.
- Mark and recapture for mobile animals: .
Prokaryotes, Eukaryotes and Viruses
- Prokaryotes: no nucleus, circular DNA in a nucleoid, 70S ribosomes, binary fission. Eukaryotes: nucleus, linear chromosomes, 80S ribosomes, membrane-bound organelles.
- Typical sizes: prokaryotes about 1–10 µm, eukaryotic cells about 10–100 µm, viruses about 20–300 nm (1 µm = 1000 nm).
- A virus = DNA or RNA inside a protein capsid, sometimes with an envelope; it can reproduce only inside a host cell.
- Lytic cycle: attachment, entry, replication, assembly, release (cell bursts).
- Endosymbiotic theory: mitochondria came from engulfed aerobic bacteria and chloroplasts from engulfed photosynthetic bacteria (cyanobacteria).
- Evidence: both organelles have circular DNA, 70S-type ribosomes, double membranes and divide like bacteria.
Protists and Fungi
- Protists are eukaryotes that are not fungi, plants or animals; they are grouped informally as animal-like, plant-like or fungus-like by how they get food.
- Amoeba moves and feeds with pseudopods, Paramecium with cilia, and Euglena with a flagellum; freshwater protists use contractile vacuoles to expel excess water.
- Most protists reproduce asexually by binary fission; Paramecium can exchange genetic material by conjugation, which adds variation without making new cells.
- Fungi are built of chitin-walled hyphae forming a mycelium, digest food outside their bodies and absorb it, and spread by spores.
- Zygomycetes make zygosporangia, ascomycetes make spores in asci, and basidiomycetes make spores on basidia.
- Fungi are key decomposers and form lichens (with algae) and mycorrhizae (with plant roots); some cause disease and Penicillium gave us penicillin.
From Algae to Flowers: Plant Diversity and Evolution
- Land plants evolved from freshwater green algae; both share chlorophyll a and b, cellulose walls and starch storage.
- Key adaptations to land: cuticle (water loss), stomata (gas exchange), vascular tissue (transport and support), pollen and seeds (reproduction without water), flowers and fruits (pollination and dispersal).
- Alternation of generations: sporophyte (2n) → meiosis → spores (n) → gametophyte (n) → mitosis → gametes (n) → fertilization → zygote (2n) → sporophyte.
- The gametophyte dominates in bryophytes; the sporophyte dominates in ferns, gymnosperms and angiosperms.
- Order of appearance: bryophytes → seedless vascular plants (ferns) → gymnosperms → angiosperms.
- Monocots: one cotyledon, parallel veins, parts in threes. Dicots: two cotyledons, net veins, parts in fours or fives.
The Animal Kingdom: Invertebrates to Vertebrates
- Animals are multicellular heterotrophs that ingest food, lack cell walls and are motile at some stage.
- Symmetry: asymmetrical (sponges), radial (cnidarians, adult echinoderms) or bilateral (most animals, with cephalization).
- Tissue layers: none (sponges), two (cnidarians) or three, with mesoderm (all other phyla).
- Body cavity: acoelomate (flatworms), pseudocoelomate (roundworms) or coelomate (annelids, molluscs, arthropods, echinoderms, chordates).
- Chordates have a notochord, dorsal hollow nerve cord, pharyngeal slits and post-anal tail at some stage of life.
- Vertebrate classes: fish, amphibians, reptiles, birds, mammals; birds and mammals are endotherms with four-chambered hearts.
Biodiversity and Human Impact
- Three levels of biodiversity: genetic (alleles within a species), species (richness and evenness), ecosystem (variety of ecosystems).
- Simpson's index of diversity: ; closer to 1 means more diverse.
- Biodiversity increases resilience through functional redundancy, complex food webs and genetic variation.
- Major threats: habitat loss, invasive species, overharvesting, pollution, monoculture and climate change.
- Genetically uniform populations, such as monocultures, can be wiped out by a single disease.
Unit 2: Evolution
The Theory of Evolution and Its Evidence
- Evolution is descent with modification: species change over generations and share common ancestors.
- Lamarck's inheritance of acquired characteristics was rejected because changes to body cells during life are not passed on in eggs and sperm.
- Homologous structures (same origin, different function) show common ancestry; analogous structures (same function, different origin) do not.
- Radiometric dating: after half-lives, the fraction of isotope remaining is ; carbon-14 has years.
- Five independent lines of evidence support evolution: fossils, biogeography, comparative anatomy, embryology and molecular biology (DNA and proteins).
Natural Selection and Adaptation
- Natural selection needs four conditions: variation, overproduction, selection (differential survival and reproduction) and inheritance.
- Fitness = relative reproductive success, not strength or speed.
- Individuals do not evolve; populations evolve as allele frequencies change over generations.
- Batesian mimicry: harmless mimics harmful. Müllerian mimicry: harmful species resemble each other.
- Resistance evolves because the antibiotic or pesticide kills susceptible individuals and leaves resistant variants that were already present to reproduce.
- Artificial selection = humans choose which individuals breed; it follows the same logic as natural selection.
Mechanisms of Evolutionary Change
- Evolution at the population level = a change in allele frequencies over generations.
- Allele frequency of = ; all allele frequencies for one gene add to 1.
- Mutation is the only source of new alleles; gene flow, drift, selection and non-random mating change the frequencies of existing alleles.
- Genetic drift is random and has its greatest effect in small populations; the bottleneck and founder effects are special cases.
- Sexual selection favours traits that increase mating success, through competition (intrasexual) or mate choice (intersexual).
Selective Breeding: Benefits and Trade-offs
- Selective breeding (artificial selection) needs variation, inheritance and human-chosen reproduction; it changes allele frequencies just as natural selection does.
- Inbreeding makes lines uniform but increases homozygosity for harmful recessive alleles, causing inbreeding depression.
- Hybridization combines traits from two lines and often produces hybrid vigour.
- Canadian examples: Marquis wheat (early ripening), canola bred from rapeseed (low erucic acid and glucosinolates), Holstein dairy cattle (milk yield).
- Monocultures of genetically uniform crops, such as the clonal Gros Michel banana, can be wiped out by a single disease.
- Seed banks preserve genetic diversity; breeding mixes genes within a species, while genetic modification inserts specific genes, possibly from other species.
Speciation and Extinction
- Biological species concept: populations that interbreed in nature to produce fertile offspring and are reproductively isolated from other groups.
- Prezygotic barriers (habitat, temporal, behavioural, mechanical, gametic) prevent zygote formation; postzygotic barriers (reduced hybrid viability, reduced hybrid fertility, hybrid breakdown) act after it.
- Allopatric speciation requires a geographic barrier; sympatric speciation occurs without one, often by polyploidy in plants.
- Adaptive radiation: one ancestor gives rise to many species filling different niches (e.g. Darwin's finches).
- Canadian risk categories: extinct, extirpated, endangered, threatened, special concern.
Unit 3: Genetic Processes
DNA, Genes, Chromosomes and Mitosis
- Nucleotide = phosphate + deoxyribose + base; A pairs with T and G pairs with C.
- Gene: a DNA segment that codes for a protein. Chromosome: one DNA molecule with its proteins. Genome: all of an organism's DNA.
- Cell cycle: G1 (growth) → S (DNA replication) → G2 (preparation) → M (mitosis and cytokinesis).
- Mitosis: prophase, metaphase, anaphase, telophase (PMAT), then cytokinesis; it produces two diploid, genetically identical cells.
- Somatic cells divide by mitosis for growth and repair; germ cells undergo meiosis to make gametes.
- In a root-tip count, % of cells in a phase ≈ % of the cycle spent in that phase.
Meiosis
- Diploid has two sets of chromosomes; haploid has one set. Humans: , .
- Meiosis I separates homologous chromosomes (reduction division); meiosis II separates sister chromatids.
- One diploid cell four genetically different haploid cells.
- Independent assortment gives chromosome combinations per gamete ( in humans).
- Crossing over happens between non-sister chromatids at chiasmata during prophase I.
- Non-disjunction in meiosis I makes all four gametes abnormal; in meiosis II, two of the four.
Mendelian Inheritance
- Law of segregation: the two alleles for a gene separate into different gametes, so each gamete carries one allele.
- gives genotypes and phenotypes dominant recessive.
- Test cross: unknown dominant homozygous recessive. Any recessive offspring means the unknown is heterozygous; gives .
- Product rule (and): . Sum rule (or): for mutually exclusive outcomes.
- Genotype is the allele combination; phenotype is the observable trait.
Beyond Simple Dominance
- Incomplete dominance: heterozygote is intermediate; gives red pink white.
- Codominance: both alleles are fully expressed in the heterozygote (roan cattle, type AB blood).
- ABO: and are codominant, both dominant to ; type O is .
- X-linked recessive: males () show the trait with one allele; females need . Fathers pass their X to every daughter and never to sons.
- gives ; for independent genes, multiply the single-gene probabilities.
Gene Mutations and Single-Gene Disorders
- A mutation is a permanent change in DNA base sequence; germline mutations are inherited, somatic mutations are not.
- Substitution: one base replaced, changing at most one codon. Insertion or deletion of a number of bases that is not a multiple of 3 causes a frameshift.
- Cystic fibrosis (CFTR gene, identified in 1989 by Lap-Chee Tsui's team in Toronto), sickle cell disease, PKU and Tay-Sachs are autosomal recessive; Huntington disease is autosomal dominant.
- Heterozygote advantage: sickle cell carriers resist severe malaria, so the allele stays common where malaria is widespread.
- Two carriers of a recessive allele (Aa × Aa) have a 1/4 chance of an affected child; an affected heterozygote with a dominant allele (Hh × hh) has a 1/2 chance.
- Treatments range from diet (PKU) and symptom management to protein-targeting drugs, transplants, gene therapy, and medicines like insulin made by engineered organisms.
Chromosomal Disorders and Genetic Technologies
- Trisomy (2n + 1) = 47 chromosomes in humans; monosomy (2n − 1) = 45. Both usually come from non-disjunction.
- Down syndrome is trisomy 21; Turner syndrome is 45,X; Klinefelter syndrome is 47,XXY.
- A karyotype arranges metaphase chromosomes in pairs by size, centromere position and banding pattern.
- Recombinant DNA: restriction enzyme cuts, DNA ligase joins a gene into a plasmid, bacteria express the protein (e.g. human insulin).
- Somatic gene therapy is not inherited; germline changes are, and Canadian law prohibits heritable changes to the human genome.
- Embryonic stem cells are pluripotent; adult stem cells are multipotent; iPSCs are adult cells reprogrammed to be pluripotent.
Unit 4: Animals: Structure and Function
The Digestive System
- Mechanical digestion (chewing, churning, emulsifying) increases surface area; chemical digestion (enzymes, acid) breaks bonds by hydrolysis.
- Carbohydrates become monosaccharides, proteins become amino acids, and lipids become fatty acids and glycerol.
- Starch digestion begins in the mouth (salivary amylase), protein digestion in the stomach (pepsin, pH about 2), and fat digestion mainly in the small intestine (lipase helped by bile).
- Villi and microvilli give the small intestine a huge surface area for absorption; sugars and amino acids enter capillaries, fats enter lacteals.
- The large intestine absorbs remaining water and houses bacteria that make vitamin K.
- Food energy: about 4 Cal/g for carbohydrate and protein and 9 Cal/g for fat.
The Respiratory System
- Air path: nasal cavity, pharynx, larynx, trachea, bronchi, bronchioles, alveoli.
- Inhalation: diaphragm and external intercostals contract, chest volume increases, lung pressure drops, air flows in.
- Gases move across the alveoli by diffusion down a concentration (diffusion) gradient; no energy is used.
- Hemoglobin in red blood cells carries almost all oxygen; each molecule can carry up to four .
- Minute ventilation = breathing rate × tidal volume; a typical resting breathing rate for healthy adults is about 12 to 20 breaths/min.
- Rising blood carbon dioxide is the main stimulus that increases breathing rate.
The Circulatory System
- Blood path: vena cavae, right atrium, tricuspid valve, right ventricle, pulmonary arteries, lungs, pulmonary veins, left atrium, bicuspid valve, left ventricle, aorta.
- Arteries carry blood away from the heart and veins carry blood toward it, regardless of oxygen content; capillary walls are one cell thick for exchange.
- The left ventricle has the thickest wall because it pumps blood through the whole systemic circuit.
- Blood is about 55% plasma; red blood cells carry oxygen, white blood cells fight infection, platelets start clotting.
- Blood pressure is systolic/diastolic, typically around 120/80 mmHg for a healthy adult; resting heart rate is typically about 60 to 100 beats/min.
- Cardiac output = heart rate × stroke volume.
Dissection: How the Systems Fit Together
- Anterior = toward the head, posterior = toward the tail, dorsal = back, ventral = belly; left and right are the specimen's own.
- Cut skin with scissors tips up, open the specimen ventral side up, and separate tissues with a blunt probe.
- The diaphragm divides the thoracic cavity (heart, lungs, trachea, esophagus, thymus) from the abdominal cavity (liver, stomach, intestines, pancreas, spleen).
- Hepatic portal system: blood from the intestines goes to the liver before the heart.
- Pulmonary circuit: right ventricle → pulmonary arteries → lungs → pulmonary veins → left atrium.
- The Three Rs of animal use: Replace, Reduce, Refine.
Breathing, Circulation and Digestion Across the Animal Kingdom
- Good respiratory surfaces are moist, thin, large in area and well supplied with blood; surface area to volume ratio falls as animals grow.
- Gas exchange: skin (earthworms, frogs), gills (fish), tracheae (insects), lungs (most land vertebrates).
- Countercurrent exchange in gills keeps a concentration gradient along the whole gill, extracting most of the oxygen from water.
- Open circulation: hemolymph bathes organs at low pressure (insects). Closed circulation: blood stays in vessels at higher pressure (earthworms, vertebrates).
- Hearts: fish 2 chambers (single circuit), amphibians 3, most reptiles 3 (partly divided), birds and mammals 4 (complete separation, supports endothermy).
- Gastrovascular cavity has one opening; a complete tract allows specialization such as the crop, gizzard and the four-chambered ruminant stomach.
System Disorders and Medical Technology
- The digestive and respiratory systems supply glucose and oxygen; the circulatory system delivers them to cells and removes carbon dioxide.
- Asthma narrows the bronchioles (inflammation, muscle tightening, mucus); emphysema destroys alveolar walls, reducing the surface area for diffusion.
- Most peptic ulcers are linked to Helicobacter pylori infection or long-term use of anti-inflammatory pain relievers, which let acid damage the stomach or duodenum lining.
- Arteriosclerosis is the hardening and narrowing of arteries; a blocked coronary artery causes a heart attack, while cardiac arrest is an electrical failure of the heartbeat.
- CT uses X-rays (ionizing radiation); MRI uses magnetic fields and radio waves; endoscopes look inside the digestive tract directly.
Unit 5: Plants: Anatomy, Growth, and Function
Plant Tissues and Structures
- Four tissue types: meristematic (divides), dermal (protects and exchanges), ground (photosynthesis, storage, support) and vascular (transport).
- Apical meristems add length (primary growth); lateral meristems such as the vascular cambium add width (secondary growth).
- Xylem carries water and minerals up from the roots in dead, hollow cells; phloem carries sugars from where they are made to where they are used, in living sieve tube cells.
- Leaf layers from top to bottom: cuticle, upper epidermis, palisade mesophyll, spongy mesophyll (with veins), lower epidermis with stomata and guard cells.
- Monocots: one cotyledon, parallel veins, scattered bundles, fibrous roots, flower parts in threes. Dicots: two cotyledons, net veins, bundles in a ring, taproot, parts in fours or fives.
- Biological drawings: with both sizes in the same unit.
Transport in Plants
- Osmosis: water diffuses across a selectively permeable membrane toward the side with the higher solute concentration.
- Root hairs actively pump in mineral ions (using ATP), so water follows by osmosis; the Casparian strip forces water through endodermal membranes.
- Cohesion-tension: transpiration creates tension, cohesion (hydrogen bonds) keeps the water column continuous, and adhesion to xylem walls helps it resist gravity.
- Guard cells take in potassium ions and water, swell, and open the stoma; they lose water and close it. Abscisic acid triggers closing during drought.
- Transpiration speeds up with brighter light, higher temperature, lower humidity and more wind.
- Pressure flow: sugar loaded at a source draws in water and raises pressure, pushing phloem sap toward a sink where sugar is unloaded.
Plant Growth and Reproduction
- Growth depends on light, water, nutrients (especially N, P and K), temperature and soil pH; the factor in shortest supply limits growth.
- Auxins: cell elongation, phototropism and gravitropism, apical dominance, root formation on cuttings. Gibberellins: stem elongation, seed germination, fruit size.
- Phototropism: auxin moves to the shaded side of the shoot, those cells elongate more, and the shoot bends toward the light.
- Stamen = anther + filament (male). Pistil = stigma + style + ovary (female). After fertilization, ovule becomes seed and ovary becomes fruit.
- Germination needs water, oxygen and a suitable temperature; imbibition starts it and the radicle emerges first.
- Asexual reproduction (cuttings, grafting, runners, tubers, bulbs) makes genetically identical clones.
Plants, Sustainability and Society
- Plants provide food, medicines, building materials, fibres and fuels, and services such as erosion control, flood control and water filtering.
- ASA (aspirin) traces back to salicin in willow bark; other plant-derived medicines include morphine (opium poppy), quinine (cinchona) and paclitaxel (Pacific yew).
- Crop rotation with legumes restores soil nitrogen (bacteria in root nodules fix nitrogen) and breaks pest cycles; seed saving keeps locally adapted, genetically diverse varieties.
- Haudenosaunee Three Sisters: corn supports the beans, bacteria in bean root nodules fix nitrogen, and squash leaves shade the soil; together they provide starch, protein and vitamins.
- Primary succession begins with no soil (pioneers: lichens and mosses); secondary succession begins where soil remains after a disturbance and proceeds faster.